FITC Annexin V Apoptosis Detection Kit II

產品名稱:FITC Annexin V Apoptosis Detection Kit II

FITC Annexin V Apoptosis Detection Kit II 產品介紹：

Material Number: 556570
Component: 51-65871A
Description: Purified Recombinant Annexin V
Size: 100 μg (1 ea)
Concentration: 0.5 mg/ml
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Component: 51-65874X
Description: FITC Annexin V
Size: 0.5 ml (1 ea)
Vol. per Test: 5 μl
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Component: 51-66211E
Description: Propidium Iodide Staining Solution
Size: 2.0 ml (1 ea)
Vol. per Test: 5 μl
Storage Buffer: Aqueous buffered solution containing no preservative.
Component: 51-66121E
Description: 10X Annexin V Binding Buffer
Size: 50 ml (1 ea)
Storage Buffer: Aqueous buffered solution containing no preservative.


FITC Annexin V Apoptosis Detection Kit II Description：

Apoptosis is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The
apoptotic program is characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment,
condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. Loss of plasma membrane is one of the earliest features.
In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma
membrane, thereby exposing PS to the external cellular environment. Annexin V is a 35-36 kDa Ca2+ dependent phospholipid-binding
protein that has a high affinity for PS, and binds to cells with exposed PS. Annexin V may be conjugated to fluorochromes including FITC.
This format retains its high affinity for PS and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing
apoptosis. Since externalization of PS occurs in the earlier stages of apoptosis, FITC Annexin V staining can identify apoptosis at an earlier
stage than assays based on nuclear changes such as DNA fragmentation.

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Store all components except the recombinant Annexin V (component no. 51-65871A) at 4°C and protected from prolonged
exposure to light. Do not freeze.
Store the recombinant Annexin V (component no. 51-65871A) at -80°C avoiding repeated freeze/thaw cycles to minimize the
risk of protein degradation.

FITC Annexin V Apoptosis Detection Kit II Product Notices：

 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results