Propidium Iodide Staining Solution

產品名稱:Propidium Iodide Staining Solution

Propidium Iodide Staining Solution 產品介紹：

Material Number: 556463
Size: 2.0 ml
Storage Buffer: Aqueous buffered solution containing no preservative.

Propidium Iodide Staining Solution Description：

The Prodidium Iodide (PI) Staining Solution may be used to assess plasma membrane (PM) integrity in Annexin V apoptosis assays. PI is a
fluorescent vital dye that stains DNA. It does not cross the PM of cells that are viable or in the early stages of apoptosis because they maintain
PM integrity. In contrast those cells in the late stages of apoptosis or already dead have lost PM integrity and are permeable to PI. PI is
detected in the orange range of the spectrum using a 562-588 nm band pass filter. Annexin V binds to cells early in apoptosis, and continues to
be bound through cell death. PI is used in two-color Annexin V flow cytometric assays to distinguish cells that are in the earlier stages of
apoptosis (Annexin V positive, PI negative) from those that are in the later stages of apoptosis or already dead (Annexin V positive, PI
positive).

Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.
This preparation contains no preservatives, thus it should be handled under aseptic conditions.
The PI Staining Solution is composed of 50 μg PI/ml in PBS (pH 7.4) and is 0.2 μm sterile filtered.

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