PE Rat Anti-Mouse CD135

產品名稱:PE 大鼠抗小鼠 CD135 抗體；PE Rat Anti-Mouse CD135

中文名稱:PE 大鼠抗小鼠 CD135 抗體

英文名稱:PE Rat Anti-Mouse CD135

PE Rat Anti-Mouse CD135 產品介紹：

Material Number: 553842
Alternate Name: FLK-2/Flt3, Ly-72
Size: 0.1 mg
Concentration: 0.2 mg/ml
Clone: A2F10.1
Immunogen: Y3 rat myeloma transfected cells
Isotype: Rat (WI) IgG2a, κ
Reactivity: QC Testing: Mouse
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.


PE Rat Anti-Mouse CD135 Description：

The A2F10 antibody reacts with Flk-2/Flt3 (Ly-72, CD135), a receptor protein tyrosine kinase closely related to c-kit, c-fms, and PDGF 
Receptor of the immunoglobulin superfamily. The Flt3 message is detected in hematopoietic stem cells and primitive progenitor cells in fetal 
liver, ***** bone marrow, and fetal and ***** thymus, as well as brain, placenta, and testis; but it is absent in more mature hematopoietic cells. 
In flow cytometric analysis, the A2F10 antibody recognizes Flt3-transfected Y3 cells (but not the parent cell line) and early B lymphoid 
lineage cells in juvenile and ***** bone marrow. A role for CD135 in the regulation of hematopoiesis is suggested by the observations that 
soluble Flk-2/Flt3 ligand can both stimulate proliferation of stem cell-enriched fetal liver, fetal thymus, and ***** bone marrow populations 
and enhance their responses to other growth factors in vitro. In addition, injection of Flk-2/Flt3 ligand stimulates extramedullary 
hematopoiesis in the mouse spleen and accumulation of dendritic cells in the hematopoietic system.10 mAb A2F10.1 is reported to 
immunoprecipitate a 150-kDa surface protein from the murine myeloblast cell line M1, which naturally expresses CD135, and to inhibit the 
binding of Flk-2/Flt3 ligand to CD135. 

PE Rat Anti-Mouse CD135 Preparation and Storage：

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.