PE Rat Anti-Mouse CD25

產品名稱:PE 大鼠抗小鼠 CD25 抗體；PE Rat Anti-Mouse CD25

中文名稱:PE 大鼠抗小鼠 CD25 抗體

英文名稱:PE Rat Anti-Mouse CD25

PE Rat Anti-Mouse CD25 產品介紹：

Material Number: 553075
Alternate Name: IL-2 Receptor α chain, p55
Size: 0.1 mg
Concentration: 0.2 mg/ml
Clone: 3C7
Immunogen: IL-2-dependent BALB/c mouse cell line
Isotype: Rat (LEW) IgG2b, κ
Reactivity: QC Testing: Mouse
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.



PE Rat Anti-Mouse CD25 Description：

The 3C7 antibody reacts with CD25, the low affinity IL-2 Receptor (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse 
strains tested. IL-2Rα by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γc (CD132) chains to 
form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express 
IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- 
Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre B-I stage 
nor on CD45R/B220high TdTsIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in 
fetal and ***** thymus. Peripheral CD25+ CD4+ T lymphocytes called regulatory T (Treg) cells are involved in the maintenance of 
self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. No. 553068). The 3C7 antibody 
recognizes an epitope of CD25 which is distinct from those recognized by mAbs 7D4 and PC61 (Cat. No. 553866), and it blocks binding of 
IL-2 to CD25.

PE Rat Anti-Mouse CD25 Preparation and Storage：

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed by gel 
filtration chromatography.
Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.