PE-Cy?7 Rat Anti-Mouse CD25

產品名稱:PE-Cy?7 大鼠抗小鼠 CD25 抗體；PE-Cy?7 Rat Anti-Mouse CD25

中文名稱:PE-Cy?7 大鼠抗小鼠 CD25 抗體

英文名稱:PE-Cy?7 Rat Anti-Mouse CD25

PE-Cy?7 Rat Anti-Mouse CD25 產品介紹：

Material Number: 552880
Alternate Name: IL-2 Receptor α chain, p55
Size: 0.1 mg
Concentration: 0.2 mg/ml
Clone: PC61
Immunogen: IL-2-dependent cytolytic mouse T-cell clone B6.1
Isotype: Rat (OFA) IgG1, λ
Reactivity: QC Testing: Mouse
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.


PE-Cy?7 Rat Anti-Mouse CD25 Description：

The PC61 antibody reacts with CD25, the low-affinity IL-2 Receptor α chain (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2Rα by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γc (CD132) chains to c form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdT- sIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and ***** thymus. Peripheral CD25+CD4+ lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. No. 553068). The PC61 antibody recognizes an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by mAbs 3C7 (Cat. No. 557364) and 7D4 (Cat. No. 553068). It blocks binding of IL-2 to CD25, presumably by inducing a conformational change in CD25.

PE-Cy?7 Rat Anti-Mouse CD25 Preparation and Storage：

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed by gel filtration chromatography.
Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.