IL1R2 / CD121b蛋白產品信息
別名: IL1R2, Il-1r2, Il1rb
表達方式:
A DNA sequence encoding the mouse IL1R2 (P27931) (Met1- Glu355) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
種屬: Mouse
表達宿主: Human Cells
IL1R2 / CD121b Protein QC Testing
純度: > 85% as determined by SDS-PAGE SDS-PAGE
生物活性:
Measured by its ability to bind biotinylated recombinant human IL1α (Cat:10128-HNCH) in a functional ELISA.
內**: < 1.0 EU per μg of the protein as determined by the LAL method
穩定性: Samples are stable for up to twelve months from date of receipt at -70℃
預測N端: Phe 14
分子量:
The recombinant mouse IL1R2 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 583 amino acids and has a predicted molecular mass of 65.1 kDa. The apparent molecular mass of the protein is approximately 91 kDa in SDS-PAGE under reducing conditions due to glycosylation.
緩沖液: Lyophilized from sterile PBS, pH7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
IL1R2 / CD121b Protein Usage Guide
儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IL1R2 / CD121b蛋白背景綜述
The pleiotropic cytokine IL1 is produced to regulate development and maintenance of the inflammatory responses, and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). A closely related receptor-like chain IL1R accessory protein (IL1RAcP) has been reported to heterodimerize with the IL1RI to form the signal transduction complex which appears to mediate all the known IL1 signal processes. IL1RII structurally consisting of a ligand binding portion comprised of three Ig -like domains, a single transmembrane region, and a short cytoplasmic domain, is expressed in a variety of cell types including B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Like the IL1RI, the human IL1RII can bind all three forms of IL1 (IL1 alpha, IL1 beta and IL1ra) but does not transmit IL1 signals. In addition to the membrane-bound form, IL1RII also exists as the soluble form, and either acts as a decoy receptor that inhibits IL1 action by blocking the binding of IL1 to the type I receptor complex. Furthermore, IL4 is reported to antagonize the activity of IL1 by inducing the expression and release of this cytokine. Recombinant soluble IL1RII is thus a potent antagonist of IL1 action, and serves as a potential therapeutic target.
參考文獻
McMahan CJ. et al., 1991, EMBO J. 10: 2821-32.
Liu C. et al., 1996, J Biol Chem. 271: 20965-72.
Rauschmayr T. et al., 1997, Proc Natl Acad Sci. 94: 5814-19.
Neumann D. et al., 2000, J Immunol. 165: 3350-7.
Colotta F. et al., 1993, Science. 261: 472-5.
規格：50ug
關 鍵 詞：Mouse IL1R2 / CD121b Protein,產品詳情，實驗室耗材，生化試劑，上海創賽，博飛美科，實驗儀器，廠家
CANSPEC品質提供的Mouse IL1R2 / CD121b Protein|廠家|產品詳情性能**，其優良的品質滿足您不同實驗的多種需求。
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