TMEM27 Protein|TMEM27蛋白質|技術資料|價格

TMEM27蛋白產品信息

別名: TMEM27
表達方式: 
A DNA sequence encoding the human TMEM27(NP_862830.1) (Met1-Pro141) was expressed with a polyhistidine tag at the C-terminus.
種屬: Human
表達宿主: Human Cells

TMEM27 Protein QC Testing

純度: (48.3+41.6) % as determined by SDS-PAGE SDS-PAGE
內**: < 1.0 EU per μg of the protein as determined by the LAL method
穩定性: Samples are stable for up to twelve months from date of receipt at -70℃
預測N端: Glu 15
分子量: 
The recombinant human TMEM27 consists of  138 amino acids and predicts a molecular mass of 15.8 KDa. It migrates as an approximately 27 and 33 KDa band in SDS-PAGE under reducing conditions.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

TMEM27 Protein Usage Guide

儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TMEM27蛋白背景綜述

TMEM27 is a membrane protein. It forms dimers and its extracellular domain is glycosylated, cleaved and shed from the plasma membrane of beta cells. Thus it has been proposed as a beta cell mass biomarker. Overexpression of TMEM27 leads to increased thymidine incorporation, whereas silencing of Tmem27 using RNAi results in a reduction of cell replication. Furthermore, transgenic mice with increased expression of Tmem27 in pancreatic beta cells exhibit increased beta cell mass. TMEM27 is important for trafficking amino acid transporters to the apical brush border of proximal tubules.

參考文獻

Pasquali L. et al., 2009, J Genet. 88 (1): 105-8.
Tosetto E. et al., 2009, Pediatr Nephrol. 24 (10): 1967-73.
Singer D. et al., 2011, Channels (Austin). 5 (5): 410-23.

規格：50ug

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