OPALIN Protein|OPALIN 蛋白質|現貨|價格

OPALIN蛋白產品信息

別名: OPALIN, HTMP10, TMEM10
表達方式: 
A DNA sequence encoding the human OPALIN (Q96PE5-2) (Thr51-Glu141) was expressed with the Fc region of mouse IgG1 at the N-terminus.
種屬: Human
表達宿主: Human Cells

OPALIN Protein QC Testing

純度: (80.7+11.2)% as determined by SDS-PAGE SDS-PAGE
內**: < 1.0 EU per μg of the protein as determined by the LAL method
穩定性: Samples are stable for up to twelve months from date of receipt at -70℃
預測N端: Asp
分子量: 
The recombinant human OPALIN/mFc  comprises 327 amino acids and has a predicted molecular mass of 37.4 kDa. The apparent molecular mass of the monomer is approximately 44 and 33 kDa in SDS-PAGE under reducing conditions due to glycosylation.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

OPALIN Protein Usage Guide

儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

OPALIN蛋白背景綜述

Opalin, or oligodendrocytic myelin paranodal and inner loop protein, is a transmembrane protein detected specifically in mammalian oligodendrocytes, and may play significant role in oligodendrocyte differentiation and myelination.Opalin has binding sites for Myt1 and cAMP-response element binding protein (CREB). Over-expression of Myt1, treatment of the cell with leukemia inhibitory factor (LIF), and cAMP analog (CREB activator) enhanced the expression of endogenous Opalin in Oli-neu cells and activated the oligodendrocyte enhancer. Thus LIF, cAMP signaling cascades and Myt1 may play significant roles in the differentiation of oligodendrocytes through their action on the Opalin oligodendrocyte enhancer. Enzymatic deglycosylation showed that myelin Opalin contained N- and O-glycans, and that the O-glycans, at least, had negatively charged sialic acids. Site-directed mutations at the glycan sites impaired the cell surface localization of Opalin. In addition to the somata and processes of oligodendrocytes, Opalin immunoreactivity was observed in myelinated axons in a spiral fashion, and was concentrated in the paranodal loop region. Immunogold electron microscopy demonstrated that Opalin was localized at particular sites in the paranodal loop membrane. These results suggest a role for highly sialylglycosylated Opalin in an intermembranous function of the myelin paranodal loops in the central nervous system.

參考文獻

Aruga J. et al., 2007, J Neurochem. 102 (5): 1533-47.
Kippert A. et al., 2008, BMC Neurosci. 9: 40.
Yoshikawa F. et al., 2008, J Biol Chem. 83 (30): 20830-40.
Golan N. et al., 2008, Glia. 56 (11): 1176-86.

規格：20ug

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