Rat CLEC4F Protein|廠家現貨|參數|產品詳情

CLEC4F蛋白產品信息

別名: CLEC4F, Clecsf13, Kclr
表達方式: 
A DNA sequence encoding the rat CLEC4F(NP_446205.1) (Arg70-Ser550) was expressed with two additional amino acids (Gly & Pro) at the N-terminus.
種屬: Rat
表達宿主: Human Cells

CLEC4F Protein QC Testing

純度: > 95 % as determined by SDS-PAGE SDS-PAGE
內**: < 1.0 EU per μg of the protein as determined by the LAL method
穩定性: Samples are stable for up to twelve months from date of receipt at -70℃
預測N端: Gly
分子量: 
The recombinant rat CLEC4F comprises 483 amino acids and predicts a molecular mass of 53.7 kDa. The apparent molecular mass of  the recombinant protein is approximately 63-68 kDa in SDS-PAGE under reducing conditions due to glycosylation.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

CLEC4F Protein Usage Guide

儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CLEC4F蛋白背景綜述

CLEC4F, a member of C-type lectins, was firstly purified from rat liver extract with high binding affinity to fucose, galactose and N-acetylgalactosamine, and un-sialylated glucosphingolipids with GalNAc or Gal terminus. However, the biological functions of CLEC4F have not been elucidated. Histochemical staining showed that mouse CLEC4F（mCLEC4F） is only expressed on F4/80+ cells localized in liver, and is undetectable in bone marrow, spleen, lymph nodes, or other tissues in ***** mice. However, mCLEC4F is detected in the liver of embryonic day 11.5 (E11.5), which is 1.5 day earlier than the formation of liver (E10) and is 3.5 day earlier than the formation of bone marrow (E15-16). Moreover, recombinant mCLEC4F.Fc binds to alpha-galactoceramide in a Ca++-dependent manner, and both galactose and ceramide can partially inhibit CLEC4F.Fc binding to alpha-galactoceramide. Interestingly, mCLEC4F-deficient (mCEC4F k/o) mice produced far less cytokines than wild type littermates after intravenous injection ofalpha-galactoceramide. This suggests that mCLEC4F is not only a specific marker for Kupffer cells, but is also critical for the presentation of glycolipid antigen to NKT cells.

參考文獻

Shie-Liang Edmond Hsieh. et al., 2009, The Journal of Immunology. 182: 78.
Ota T. et al., 2004, Nat Genet. 36 (1): 40-5.
Bonaldo MF. et al., 1996, Genome Res. 6 (9): 791-806.

規格：50ug

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