PDGFRB / PDGFR-1 / CD140b蛋白產品信息
別名:
PDGFRβ, DGFRB, CD140B, JTK12, PDGFR, PDGFR1
表達方式:
A DNA sequence encoding the extracellular domain of human PDGFRβ (NP_002600.1) (Met 1- Lys 531) was expressed with six amino acids (ENLYFQ) at the C- terminus.
種屬: Human
表達宿主: Human Cells
PDGFRB / PDGFR-1 / CD140b Protein QC Testing
純度: > 95 % as determined by SDS-PAGE SDS-PAGE
內**: < 1.0 EU per μg of the protein as determined by the LAL method
穩定性: Samples are stable for up to twelve months from date of receipt at -70℃
預測N端: Leu 33
分子量:
The recombinant human PDGFRβ consists of 506 amino acids and predicts a molecular mass of 57.1 KDa. It migrates as an approximately 92-116 KDa band in SDS-PAGE under reducing conditions.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
PDGFRB / PDGFR-1 / CD140b Protein Usage Guide
儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
PDGFRB / PDGFR-1 / CD140b蛋白背景綜述
Beta-type platelet-derived growth factor receptor, also known as CD140b, PDGFRB, PDGF-R-beta, PDGF receptor-β, is a member of the protein kinase superfamily and CSF-1/PDGF receptor subfamily. The PDGF family consists of PDGF-A, -B, -C and -D, which form either homo- or heterodimers (PDGF-AA, -AB, -BB, -CC, -DD). The four PDGFs are inactive in their monomeric forms. The PDGFs bind to the protein tyrosine kinase receptors PDGF receptor-α and -β. These two receptor isoforms dimerize upon binding the PDGF dimer, leading to three possible receptor combinations, namely -αα, -ββ and -αβ. The extracellular region of the PDGF receptor-β consists of five immunoglobulin-like domains while the intracellular part is a tyrosine kinase domain. The ligand-binding sites of PDGF receptor-α and -β are located to the three first immunoglobulin-like domains. PDGF-CC specifically interacts with PDGFR-αα and -αβ, but not with –ββ, and thereby resembles PDGF-AB. PDGF-DD binds to PDGFR-ββ with high affinity, and to PDGFR-αβ to a much lower extent and is regarded as PDGFR-ββ specific. PDGF-AA binds only to PDGFR-αα, while PDGF-BB is the only PDGF that can bind all three receptor combinations with high affinity.
參考文獻
Chi KD. et al., 1997, Oncogene. 15: 1051-7.
Arcaro A. et al., 2000, Mol Cell Biol. 20: 3817-30.
Bergsten E. et al., 2001, Nat Cell Biol. 3: 512-16.
Zhang Z. et al., 2004, Protein Sci. 13: 2819-24.
Morerio C. et al., 2004, Cancer Res. 64: 2649-51.
規格：100ug
關 鍵 詞：PDGFRB / PDGFR-1 / CD140b Protein,價格,使用說明 ，實驗室耗材，生化試劑，上海創賽，博飛美科，實驗儀器，廠家
CANSPEC品質提供的PDGFRB / PDGFR-1 / CD140b Protein|價格|使用說明 性能**，其優良的品質滿足您不同實驗的多種需求。
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