CD40LG / CD40L / TNFSF5蛋白產品信息
別名: CD40LG, CD40L, TNFSF5
表達方式:
A DNA sequence encoding the cynomolgus CD40LG (F6Q0N6) (Met113-Leu261) was expressed, with the Fc region of human IgG1 at the C-terminus.
種屬: Cynomolgus
表達宿主: Human Cells
CD40LG / CD40L / TNFSF5 Protein QC Testing
純度: > 85 % as determined by SDS-PAGE SDS-PAGE
內**: < 1.0 EU per μg of the protein as determined by the LAL method
穩定性: Samples are stable for up to twelve months from date of receipt at -70℃
預測N端: Glu
分子量:
The recombinant cynomolgus CD40LG is a disulfide-linked homodimer. The reduced monomer comprises 409 amino acids and has a calculated molecular mass of 44.7 KDa.The apparent molecular mass of the protein is approximately 54 KDa respectively in SDS-PAGE.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
CD40LG / CD40L / TNFSF5 Protein Usage Guide
儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CD40LG / CD40L / TNFSF5蛋白背景綜述
CD40 ligand, also known as CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5, CD154, CD40L and TNFSF5, is a single-pass type II membrane protein which belongs to the tumor necrosis factor family. CD40L ( CD154 / TNFSF5 ) is specifically expressed on activated CD4+ T-lymphocytes. The soluble form of CD40L ( CD154 / TNFSF5 ) is derived from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. CD40L ( CD154 / TNFSF5 ) mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. It is involved in immunoglobulin class switching. Defects in CD40L are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1), also known as X-linked hyper IgM syndrome (XHIM). It is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes.
參考文獻
Korthaeuer U. et al., 1993, Nature. 361: 539-41.
Lin Q. et al., 1996, J Clin Invest. 97: 196-201.
Seyama K. et al.,1998, Blood. 92: 2421-34.
Khandekar SS. et al., 2001, Protein Expr Purif. 23: 301-10.
Furman MI. et al., 2004, J Am Coll Cardiol. 43: 2319-25.
規格：50ug
關 鍵 詞：Cynomolgus CD40LG / CD40L / TNFSF5 Protein,價格,參數,廠家，實驗室耗材，生化試劑，上海創賽，博飛美科，實驗儀器，廠家
CANSPEC品質提供的Cynomolgus CD40LG / CD40L / TNFSF5 Protein|價格|參數|廠家性能**，其優良的品質滿足您不同實驗的多種需求。
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