Novel coronavirus (HCoV-EMC/2012) Spike protein S1 (aa 1-725) |價格|參數

S protein (Human betacoronavirus 2c EMC/2012)蛋白產品信息

別名: S
表達方式: 
A DNA sequence encoding the spike protein S1 (Human betacoronavirus 2c EMC/2012)( AFS88936.1)(Met1-Glu725) was fused with a polyhistidine tag at the C-terminus.
種屬: Human betacoronavirus
表達宿主: Human Cells

S protein (Human betacoronavirus 2c EMC/2012) Protein QC Testing

純度: > 95 % as determined by SDS-PAGE SDS-PAGE
生物活性:
1. Measured by its binding ability in a functional ELISA. Immobilized Spike protein S1 (aa 1-725) (Cat: 40069-V08H) at 10 μg/ml (100 μl/well) can bind biotinylated DPP4 (Cat: 10688-HNCH). The EC50 of of biotinylated DPP4 (Cat: 10688-HNCH) is 0.6-1.4 μg/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized Spike protein S1 (aa 1-725) (Cat: 40069-V08H) at 10 μg/ml (100 μl/well) can bind biotinylated Fc-DPP4 (Cat: 10688-H01H). The EC50 of biotinylated Fc-DPP4 (Cat: 10688-H01H) is 0.02-0.04 μg/ml.
內**: < 1.0 EU per μg of the protein as determined by the LAL method
穩定性: Samples are stable for up to twelve months from date of receipt at -70℃
預測N端: Tyr 18
分子量: 
The recombinant spike protein S1 (Human betacoronavirus 2c EMC/2012) comprises 719 amino acids and has a predicted molecular mass of 79.9 kDa. It migrates as an approximately 94 kDa band in SDS-PAGE under reducing conditions.
緩沖液: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

S protein (Human betacoronavirus 2c EMC/2012) Protein Usage Guide

儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

S protein (Human betacoronavirus 2c EMC/2012)蛋白背景綜述

The spike protein is one of the four major structural proteins of the coronavirus. Coronavirus entry is mediated by the viral spike protein. The 180-kDa oligomeric spike protein of the murine coronavirus mouse hepatitis virus strain A59 is posttranslationally cleaved into an S1 receptor binding unit and an S2 membrane fusion unit. Both biochemical and functional data show that the coronavirus spike protein is a class I viral fusion protein.
HCoV-EMC is a novel coronavirus isolated from a Saudi patient presenting with pneumonia and renal failure in June 2012. Genome sequencing showed that this virus belongs to the group C species of the genus betacoronavirus and phylogenetically related to the bat coronaviruses HKU4 and HKU5 previously found in lesser bamboo bat and Japanese Pipistrelle bat of Hong Kong respectively. HCoV-EMC has been identified as a highly lethal virus that could be passed from animals to humans very easily. The virus, like other coronaviridae, has the potential to infect bats and pigs in addition to humans. HCoV-EMC is related to the causative agent of SARS (severe acute respiratory syndrome), SARS coronavirus. and also may be even more infectious as it does not use the human receptor protein ACE2. The receptor used by HCov-EMC has not been identified. Symptoms of infection include kidney failure and severe pneumonia. The animal source has still not been identified but may circulate among populations of bats.

參考文獻

Sander van Boheemen. et al., 2012, mBio. 3 (6): e00473-12.
Muller M A. et al., 2012, mBio 3 (6): e00515-12.
Chan JF. et al., 2012, J Infect. 65 (6): 477-89.

規格：20ug

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