Biotinidase / Biotinase / BTD Protein|技術資料|價格

Biotinidase / Biotinase / BTD蛋白產品信息

別名: BTD
表達方式: 
A DNA sequence encoding the human BTD (P43251) (Met1-Asp543) with a C-terminal polyhistidine tag was expressed.
種屬: Human
表達宿主: Human Cells

Biotinidase / Biotinase / BTD Protein QC Testing

純度: > 95 % as determined by SDS-PAGE SDS-PAGE
內**: < 1.0 EU per μg of the protein as determined by the LAL method
穩定性: Samples are stable for up to twelve months from date of receipt at -70℃
預測N端: Ala 42
分子量: 
The recombinant human BTD comprises 513 amino acids and has a predicted molecular mass of 58.2 kDa. The apparent molecular mass of the protein is approximately 66-76 kDa in SDS-PAGE under reducing conditions.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

Biotinidase / Biotinase / BTD Protein Usage Guide

儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Biotinidase / Biotinase / BTD蛋白背景綜述

Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing of fats, carbohydrates, and proteins. Biotin is attached to these carboxylase enzymes through an amino acid (the building material of proteins) called lysine, forming a complex calledbiocytin.

參考文獻

Pindolia K. et al. 2010, Human mutation. 31 (9): 983-91.
Dobrowolski SF. et al., 2003, Mol Genet Metab. 78 (2): 100-7.
McMahon RJ. 2002, Annu Rev Nutr. 22: 221-39.

規格:10ug

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