Cynomolgus NKp80 / KLRF1 Protein |現貨|技術資料

NKp80 / KLRF1蛋白產品信息

別名: KLRF1
表達方式: 
A DNA sequence encoding the cynomolgus KLRF1(Q8MI05) (Val66-Tyr231) was expressed with the Fc region of human IgG1 at the C-terminus.
種屬: Cynomolgus
表達宿主: Human Cells

NKp80 / KLRF1 Protein QC Testing

純度: > 90 % as determined by SDS-PAGE SDS-PAGE
內**: < 1.0 EU per μg of the protein as determined by the LAL method
穩定性: Samples are stable for up to twelve months from date of receipt at -70℃
預測N端: Glu
分子量: 
The recombinant cynomolgus KLRF1 comprises 426 amino acids and has a calculated molecular mass of 47.8 KDa.The apparent molecular mass of the protein is approximately 59 KDa respectively in SDS-PAGE.
緩沖液: Lyophilized from sterile PBS, pH7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

NKp80 / KLRF1 Protein Usage Guide

儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

NKp80 / KLRF1蛋白背景綜述

NKp80, also known as KLRF1, is an activating homodimeric C-type lectin-like receptor which is expressed on nearly all natural killer cells and stimulates their cytoxicity and cytokine release. NKp80 stimulates cytotoxicity upon engagement of its genetically linked ligand: myeloid-specific CTLR activation-induced C-type lectin (AICL). NKp80, but not NKp80 mutated at tyrosine 7 (NKp80/Y7F), is tyrosine phosphorylated. Accordingly, NKp80/Y7F, but not NKp80/Y30F or NKp80/Y37F, failed to induce cytotoxicity. NKp80 phosphopeptides comprising the hemi-ITAM-like sequence surrounding tyrosine 7 bound Lck- and Syk-family kinases; accordingly, cross-linking of NKp80, but not NKp80/Y7F, induced Syk phosphorylation. Moreover, inhibition of Syk kinase, but not ZAP-70 kinase, impaired cytotoxic responses through NKp80. Atypical residues in the hemi-ITAM-like motif of NKp80 cause an altered stoichiometry of phosphorylation but did not substantially affect NK cytotoxicity. Altogether, these results show that NKp80 uses an atypical hemi-ITAM and Syk kinase to trigger cellular cytotoxicity.

參考文獻

Kuttruff S. et al., 2009, Blood. 113 (2): 358-69.
Dennehy KM. et al., 2011, J Immunol. 186 (2): 657-61.
Roda-Navarro P. et al., 2000, Eur J Immunol. 30 (2): 568-76.

規格：50ug

關 鍵 詞：Cynomolgus NKp80 / KLRF1 Protein ,現貨,技術資料，實驗室耗材，生化試劑，上海創賽，博飛美科，實驗儀器，廠家

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