IL6ST / gp130蛋白產品信息
別名: IL6ST
表達方式:
A DNA sequence encoding the cynomolgus IL6ST (NP_005214.2) (Met1-Ile618) was expressed with the Fc region of human IgG1 at the C-terminus.
種屬: Cynomolgus
表達宿主: Human Cells
IL6ST / gp130 Protein QC Testing
純度: > 85 % as determined by SDS-PAGE SDS-PAGE
內**: < 1.0 EU per μg of the protein as determined by the LAL method
穩定性: Samples are stable for up to twelve months from date of receipt at -70℃
預測N端: Glu 23
分子量:
The recombinant cynomolgus IL6ST is a disulfide-linked homodimer. The reduced monomer comprises 837 amino acids and has a calculated molecular mass of 94.8 KDa.The apparent molecular mass of the protein is approximately 116 KDa respectively in SDS-PAGE.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
IL6ST / gp130 Protein Usage Guide
儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IL6ST / gp130蛋白背景綜述
Glycoprotein 130 (gp130), also known as IL6ST, IL6R-beta and CD130, is a common signal-transducing molecule shared by the functional receptor complexes of the IL-6 family of cytokines (IL-6, IL-11, leukemia inhibitory factor (LIF), granulocyte colonystimulating factor, ciliary neurotrophic factor, and cardiotrophin-1). It belongs to the class a…? cytokine receptor family, and was found in tissues such as brain, heart, thymus, spleen, kidney, lung and liver. Mouse gp130 contains five fibronectin type-III domains, and one Ig-like C2-type (immunoglobulin-like) domain. gp130 is an important part of many different types of signaling complexes. It binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. This complex of 3 proteins then homodimerizes to form a hexameric complex which can produce downstream signals. Inactivation of gp130 is lethal to mice. The mice homozygous for a knock-in mutation in gp130 ( Y757F/Y757F ) that leads to gp130-dependent signal transducers and activators of transcription (STAT) 1/3 hyperactivation develop a broad spectrum of hematopoietic abnormalities, including splenomegaly, lymphadenopathy, and thrombocytosis. gp130 is also involved in various human diseases, such as liver disease, coronary artery disease and myocardial infarction. Furthermore, gp130 is widely expressed in breast cancer cell lines and primary tumors, thus it have been implicated in constitutive STAT3 activation in breast cancer.
參考文獻
Saito M. et al., 1992, J Immunol. 148 (12): 4066-71.
Murakami M. et al., 1993, Science. 260 (5115): 1808-10.
Yoshida K. et al., 1996, Proc Natl Acad Sci. 93 (1): 407-11.
Selander K.S. et al., 2004, Cancer Res. 64 (19): 6924-33.
Jenkins BJ. et al., 2005, Blood. 105 (9): 3512-20.
Luchtefeld M. et al., 2007, J Exp Med. 204 (8): 1935-44.
規格：100ug
關 鍵 詞：Cynomolgus IL6ST / gp130 Protein ,使用說明,現貨，實驗室耗材，生化試劑，上海創賽，博飛美科，實驗儀器，廠家
CANSPEC品質提供的Cynomolgus IL6ST / gp130 Protein |使用說明|現貨性能**，其優良的品質滿足您不同實驗的多種需求。
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