E-cadherin / CDH1 / Cadherin-1 / E-Cad蛋白產品信息
別名: E-Cad, CDH1,Arc-1, CD324, CDHE, ECAD, LCAM, UVO
表達方式:
A DNA sequence encoding the human E-Cad (P12830)(Met1-Ile707) was expressed with the Fc region of human IgG1 at the C-terminus.
種屬: Human
表達宿主: Human Cells
E-cadherin / CDH1 / Cadherin-1 / E-Cad Protein QC Testing
純度: > 90 % as determined by SDS-PAGE SDS-PAGE
內**: < 1.0 EU per μg of the protein as determined by the LAL method
穩定性: Samples are stable for up to twelve months from date of receipt at -70℃
預測N端: Gln 23 & Asp 155
分子量:
The recombinant human E-Cad/Fc is a disulfide-linked homodimer. The reduced monomer comprises 791 amino acids and has a predicted molecular mass of 87.1 kDa. The apparent molecular mass of the protein is approximately 116-126 kDa in SDS-PAGE under reducing conditions.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
E-cadherin / CDH1 / Cadherin-1 / E-Cad Protein Usage Guide
儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
E-cadherin / CDH1 / Cadherin-1 / E-Cad蛋白背景綜述
Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is the classical cadherin molecule and contains five cadherin domains of ~110 aa each in the extracellular domain. E-Cad is expressed in the non-neural epithelial tissues as disulfide-linked homodimer, and plays a key role in the organization and integrity of most epithelial tissues, cell differentiation and tissue development. CDH1 interacts directly, via the cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion complex which connects to the actin skeleton through the actin binding of alpha-catenin. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas (gastric, breast, ovary, endometrium and thyroid).
參考文獻
Berx G. et al., 1995, Genomics. 26: 281-9.
Yoshiura K. et al., 1995, Proc Natl Acad Sci. 92: 7416-9.
Becker K-F. et al., 1994, Cancer Res. 54: 3845-52.
Marambaud P. et al., 2002, EMBO J. 21: 1948-56.
Steinhusen U. et al., 2001, J Biol Chem. 276: 4972-80.
規格：50ug
關 鍵 詞：E-cadherin / CDH1 / Cadherin-1 / E-Cad Protein,使用說明,價格，實驗室耗材，生化試劑，上海創賽，博飛美科，實驗儀器，廠家
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