PLBD2 Protein|PLBD2蛋白質|技術資料|廠家現貨

PLBD2蛋白產品信息

別名: PLBD2
表達方式: 
A DNA sequence encoding the human PLBD2 (Q8NHP8) (Met1-Asp589) was expressed with a polyhistidine tag at the C-terminus.
種屬: Human
表達宿主: Human Cells

PLBD2 Protein QC Testing

純度: (69.5+23.3+6.5) % as determined by SDS-PAGE SDS-PAGE
內**: < 1.0 EU per μg of the protein as determined by the LAL method
穩定性: Samples are stable for up to twelve months from date of receipt at -70℃
預測N端: Ile 42
分子量: 
The recombinant human PLBD2 consists of  559 amino acids and predicts a molecular mass of  62.7 KDa. It migrates as an approximately 68,46 and 34 KDa band in SDS-PAGE under reducing conditions.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

PLBD2 Protein Usage Guide

儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PLBD2蛋白背景綜述

PLBD2 localizes to the lysosome, as its absence could plausibly lead to a serious yet unrecognized lysosomal storage disease. PLBD1 and PLBD2 are semi-orphans in the sense of being probable phospholipases of B class but with uncertain physiological substrates and thus functionalities. PLBD1 and PLBD2 constitute a small gene family (sequence homology class) within vertebrates though one that occurs expanded in some early diverging eukaryotes. PLBD2 presents a special difficulty in that a sequence of post-translational steps are apparently necessary for its activation. Without these, potential substrates can hardly be assayed. These steps include removal of the signal peptide, mannosylation appropriate to the lysosome targeting receptor, and self-catalytic proteolytic activation to expose the substrate binding site as this becomes appropriate.

參考文獻

Morgan CP. et al., 2004), Biochem J. 382 (2): 441-9.
Kim W. et al., 2011, Mol Cell. 44 (2): 325-40.
Havugimana PC. et al., 2012. Cell. 150 (5): 1068-81.

規格：50ug

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