EFNB2 / EphrinB2蛋白產品信息
別名: EFNB2
表達方式:
A DNA sequence encoding the canine EFNB2 (B0LDS6) (Met1-Ala229) was expressed with the Fc region of human IgG1 at the C-terminus.
種屬: Canine
表達宿主: Human Cells
EFNB2 / EphrinB2 Protein QC Testing
純度: (93.5+2.7) % as determined by SDS-PAGE SDS-PAGE
內**: < 1.0 EU per μg of the protein as determined by the LAL method
穩定性: Samples are stable for up to twelve months from date of receipt at -70℃
預測N端: Ile 28
分子量:
The recombinant canine EFNB2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 443 amino acids and has a predicted molecular mass of 49.2 kDa. The apparent molecular mass of the protein is approximately 58 and 35 kDa in SDS-PAGE under reducing conditions.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
EFNB2 / EphrinB2 Protein Usage Guide
儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
EFNB2 / EphrinB2蛋白背景綜述
The ephrins (Eph) and Eph receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosyl- phosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. All ephrins share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The cytoplasmic domains are approximately 80 amino acids long and are highly conserved, especially the last 33 amino acids. In molecular biology, ephrins and Eph receptors are components of cell signaling pathways involved in animal development, and implicated in some cancers. Eph receptors are classified as receptor tyrosine kinases (RTKs), and form the largest sub-family of RTKs. Almost all Eph receptors are expressed during various well defined stages of development in assorted locations and concentrations. Ephrin-B2 is a member of the ephrin ligand family, which binds members of the Eph receptor family. It has been shown to bind EphA4, EphB1, EphB2, EphB3, and EphB4. Ephrin-B2 plays a role in a variety of developmental processes, including arterial-venous differentiation, neural crest cell migration, and axon guidance.
參考文獻
Cerretti DP. et al., 1995, Mol Immunol. 32 (16): 1197-205.
Gale NW. et al.,1996, Neuron. 17 (1): 9-19.
Flanagan J. et al., 1998, Annual Review of Neuroscience. 21: 309-45.
Holder N. et al., 1999, Development. 126 (10): 2033-44.
Wilkinson DG. et al., 2001, Nat Rev Neurosci. 2 (3): 155-64.
Murai KK. et al., 2003, Journal of Cell Science. 116: 2823-32.
規格：50ug
關 鍵 詞：Canine EFNB2 / EphrinB2 Protein,技術資料,參數，實驗室耗材，生化試劑，上海創賽，博飛美科，實驗儀器，廠家
CANSPEC品質提供的Canine EFNB2 / EphrinB2 Protein|技術資料|參數性能**，其優良的品質滿足您不同實驗的多種需求。
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