H7N9 (A/Anhui/1/2013) HA / Hemagglutinin Protein Product Information
Synonym : HA
Protein Construction:
A DNA sequence encoding the Influenza A virus (A/Anhui/1/2013(H7N9)) hemagglutinin (AGJ51953.1) (Met1-Val524) (cleavage between Arg339 and Gly440, HA1+HA2) was expressed with a C-terminal polyhistidine tag.
Source: Influenza A virus
Expression Host: Human Cells
H7N9 (A/Anhui/1/2013) HA / Hemagglutinin Protein QC Testing
Purity: (53.8+44.7) % as determined by SDS-PAGE SDS-PAGE
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
Stability: Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal: Asp 19
Molecular Mass:
The recombinant hemagglutinin of Influenza A virus (A/Anhui/1/2013(H7N9)) comprises 517 amino acids and has a predicted molecular mass of 57.6 kDa. The apparent molecular mass of the protein is approximately 43 and 27 kDa in SDS-PAGE under reducing conditions.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
H7N9 (A/Anhui/1/2013) HA / Hemagglutinin Amino Acid Sequence
DKICLGHHAV SNGTKVNTLT ERGVEVVNAT ETVERTNIPR ICSKGKRTVD LGQCGLLGTI TGPPQCDQFL EFSADLIIER REGSDVCYPG KFVNEEALRQ ILRESGGIDK EAMGFTYSGI RTNGATSACR RSGSSFYAEM KWLLSNTDNA AFPQMTKSYK NTRKSPALIV WGIHHSVSTA EQTKLYGSGN KLVTVGSSNY QQSFVPSPGA RPQVNGLSGR IDFHWLMLNP NDTVTFSFNG AFIAPDRASF LRGKSMGIQS GVQVDANCEG DCYHSGGTII SNLPFQNIDS RAVGKCPRYV KQRSLLLATG MKNVPEIPKG RGLFGAIAGF IENGWEGLID GWYGFRHQNA QGEGTAADYK STQSAIDQIT GKLNRLIEKT NQQFELIDNE FNEVEKQIGN VINWTRDSIT EVWSYNAELL VAMENQHTID LADSEMDKLY ERVKRQLREN AEEDGTGCFE IFHKCDDDCM ASIRNNTYDH SKYREEAMQN RIQIDPVKLS SGYKDVAHHH HHHHHHH
H7N9 (A/Anhui/1/2013) HA / Hemagglutinin Protein Usage Guide
Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
H7N9 (A/Anhui/1/2013) HA / Hemagglutinin Protein Description
H7N9 is a subtype of Influenza virus A. On April 1, 2013, the World Health Organization (WHO) first reported 3 human infections with a new influenza A (H7N9) virus in China. Since then, additional cases have been reported. This new H7N9 virus is an avian (bird) influenza (flu) virus. Influenza (flu) is a respiratory infection in mammals and birds. The virus is divided into three main types (Influenza A, Influenza B, and Influenza C), which are distinguished by differences in two major internal proteins (hemagglutinin (HA) and neuraminidase (NA).
Influenza A is further divided into subtypes based on differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA), which are the most important targets for the immune system. The notation HhNn is used to refer to the subtype comprising the hth discovered Hemagglutinin (HA) protein and the nth discovered neuraminidase (NA) protein. The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.
The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA0, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA0) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
References
Barman S. et al., 2000, J Virol. 74: 6538-45.
Suzuki T. et al., 2005, J Virol. 79: 11705-15.
Shinya K. et al., 2006, Nature. 440 (7083): 435-6.
Marjuki H. et al., 2006, J Biol Chem. 281: 16707-15.
Christophe F. et al., 2009, Science. 324: 1557-61.
Von Itzstein M. 2007, Nat Rev Drug Discov. 6: 967-74.
規格：50ug
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