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    產品資料

    細胞解離產品

    產品名稱: 細胞解離產品
    產品型號: C14-13150-016
    產品展商: GIBCO
    產品文檔: 無相關文檔

    簡單介紹

    細胞解離液Cell dissociation buffer 不含酶,由Hank’s液配制 保存:15-30°


    細胞解離產品  的詳細介紹
    詳細說明:
    Cell Dissociation Buffers are membrane-filtered, isotonic, and enzyme-free aqueous formulations of salts, chelating agents, and cell-conditioning agents in either Ca2+- and Mg2+-free Hanks balanced salt solution or Ca2+- and Mg2+-free phosphate-buffered saline. They are suitable for gentle dissociation of mammalian cells from support substrates and each other, for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry (1,2). These buffers are not recommended for routine passage of strongly adherent cell types.

    Performance and quality testing: Buffers are membrane filtered and mycoplasma tested. Cell integrity after dissociation with buffers is measured with a plating efficiency assay and a serotonin receptor function assay.

    常用規格

    Chelators:

    EDTA 

    Product Size:

    100 ml 

    Classification:

    Animal Origin-Free 

    Enzyme Stability:

    Room Temperature Stable 

    Phenol Red Indicator:

    No Phenol Red 


    相關信息:

    品名

    產品描述

    貨號

    規格

    細胞解離液

    Cell dissociation buffer

    不含酶,由Hank’s液配制

    保存:15-30°

    13150-016

    100ml

    細胞解離液

    Cell dissociation buffer

    不含酶,由PBS配制

    保存:15-30°

    13151-014

    100ml

    膠原酶,collagenase

    活性:每毫克不少于125U,2-8°

    17018-029

    500mg

    I型膠原酶,collagenase Type I

    可解離組織,建議用于上皮、肺、脂肪和腎上腺組織細胞,2-8°保存

    17100-017

    1g

    II型膠原酶,collagenase Type II

    可解離組織,建議用于肝、骨、甲狀腺、心和唾液腺組織細胞,2-8°保存

    17101-015

    1g

    IV型膠原酶,collagenase Type IV

    可解離胰腺組織,用于分離胰島,

    2-8°保存

    17104-019

    1g

    分散酶,Dispase

    溫和的解離組織得到單個細胞,

    2-8°保存

    17105-041

    5g

    胰酶替代物,TrypLE Select

    配方包含1mM EDTADPBS,

    不含酚紅,保存:2-8°

    12563-011

    100ml

     

    12563-029

    500ml

    胰酶替代物,TrypLE Express

    配方包含1mM DPBS,

    含酚紅,保存:2-8°

    12605-010

    100ml

     

    12605-028

    500ml

    胰酶替代物,TrypLE Express

    配方包含1mMDPBS,

    不含酚紅,保存:2-8°

    12604-013

    100ml

     

    12604-021

    500ml

    胰酶,Trpsin,0.25%

    不含鈣鎂離子,含酚紅

    保存:-5 - -20°

    15050-065

    100ml

     

    15050-057

    500ml

    胰酶,Trpsin,2.5%10X

    無菌條件下以Hank’s10倍稀釋,

    不含鈣鎂離子,保存:-5 - -20°

    15090-046

    100ml

    胰酶-EDTA,

    0.05%胰酶,EDTA。4Na

    不含鈣鎂離子,含酚紅

    保存:-5 - -20°

    25300-054

    100ml

    25300-062

    500ml

    胰酶-EDTA,(10X

    0. 5%胰酶,EDTA。4Na

    無菌條件下以Hank’s10倍稀釋,

    不含鈣鎂離子,保存:-5 - -20°

    15400-054

    100ml

    胰酶-EDTA,

    0.25%胰酶,EDTA。4Na

    不含鈣鎂離子,含酚紅

    保存:-5 - -20°

    25200-056

    100ml

    25200-072

    500ml

    胰酶(1250),干粉

    保存:2-8°

    27250-018

    100g

    胰酶抑制劑,大豆

    0.25-0.5mg/mlPBS溶液滅活等體積胰酶,保存:-5 - -20°

    17075-029

    1g

    EDTA,Versene,15000

    0.2g/l EDTA.4Na磷酸緩沖鹽溶液,

    =0.48Mm,保存:2-8°

    15040-066

    100ml

    Low Trypsin-High EDTA,

    0.025% 胰酶, 0.75mM EDTA (1X), 溶于PBS,不含鈣鎂離子

    SM-2004-C

    100ml

    Trypsin 0.25%

    Hank‘s 液,不含鈣鎂離子

    SM-2001-C

    100ml

    Accutase

     

    SCR005

    100ml

    Accumax

     

    SCR006

    100ml

    Trypsin-EDTA

    0.05% Trypsin & 0.53mM EDTA,

    溶于Hank‘s液,不含鈣鎂

    SM-2002-C

    100ml

    Trypsin-EDTA

    0.25% Trypsin & 1mM EDTA,

    溶于Hank‘s液,不含鈣鎂離子

    SM-2003-C

    100ml




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